QF PCR - Prenatal

QF-PCR Prenatal Testing

Quantitative Fluorescent PCR (QF-PCR) is an advanced prenatal testing method used to detect common chromosomal aneuploidies quickly and accurately. As a PCR-based technique, QF-PCR amplifies specific DNA markers on the chromosomes of interest to determine the number of chromosome copies in fetal DNA extracted from the mother‘s blood. This technique is especially valuable for high-risk pregnancies predicted by non-invasive prenatal screening (NIPS) or serum screening, offering a rapid and economical approach for prenatal diagnosis.

With its ability to deliver fast results and high accuracy, QF-PCR is an essential tool in modern prenatal care, helping healthcare providers and expectant parents make informed decisions about pregnancy management.

QF-PCR involves the amplification of polymorphic markers located on specific chromosomes to detect aneuploidies. The process begins with extracting fetal DNA from a maternal blood sample, a non-invasive procedure that poses no risk to the fetus. This DNA is then analyzed using QF-PCR to measure the quantity of specific chromosomal markers.

Here’s a step-by-step breakdown of the QF-PCR process:

  1. Sample Collection: A blood sample is taken from the pregnant woman. This sample contains fetal DNA that has crossed over from the placenta into the maternal bloodstream.

  2. DNA Extraction: The fetal DNA is isolated from the maternal blood sample in the laboratory.

  3. PCR Amplification: The QF-PCR technique amplifies specific DNA markers associated with chromosomes of interest. This amplification process is performed using fluorescent dyes, which make the markers visible for analysis.

  4. Detection and Analysis: The amplified DNA markers are analyzed to determine the number of copies of chromosomes present. Abnormalities in the number of copies can indicate chromosomal aneuploidies.

  5. Result Interpretation: The results are reviewed to identify any chromosomal abnormalities, such as trisomies (extra chromosomes). The findings help guide further diagnostic and clinical decisions.

QF-PCR is highly effective in detecting common chromosomal aneuploidies, including:

While QF-PCR provides targeted analysis of specific chromosomal abnormalities, it does not replace comprehensive tests like karyotyping or chromosomal microarray analysis (CMA) if more detailed information is needed. However, it offers a rapid and cost-effective method for initial screening.


Clinical Utility

QF-PCR is a valuable tool in prenatal diagnostics, providing several key benefits for clinical decision-making:

  1. Rapid Results: One of the major advantages of QF-PCR is its quick turnaround time, typically providing results within a few days. This speed is crucial for managing high-risk pregnancies and making timely decisions about further testing or pregnancy management.

  2. Effective Detection of Common Aneuploidies: QF-PCR is highly effective in detecting the most common chromosomal aneuploidies, including trisomy 21, 18, and 13. Its high sensitivity and specificity ensure accurate identification of these conditions, which are associated with significant developmental and health issues.

  3. Targeted Analysis: The technique focuses on specific DNA markers related to the chromosomes of interest. This targeted approach allows for precise detection of common chromosomal abnormalities, minimizing the risk of false positives or negatives.

  4. High Sensitivity and Specificity: QF-PCR demonstrates excellent sensitivity and specificity in detecting numerical chromosomal abnormalities. This reliability is crucial for making informed decisions about pregnancy management and potential interventions.

  5. Guidance for Further Testing: Positive QF-PCR results for chromosomal abnormalities can guide the need for additional diagnostic tests, such as karyotyping or CMA, for a more comprehensive evaluation of the fetal chromosomal profile.


When to Consider QF-PCR Prenatal Testing

QF-PCR is recommended in several scenarios where rapid and accurate detection of chromosomal abnormalities is necessary:


MapmyGenome Offerings

At MapmyGenome, we offer advanced QF-PCR prenatal testing to support expectant parents and healthcare professionals in managing high-risk pregnancies. Our QF-PCR testing provides:

Test specification

Technique

Variant types

TAT

Sample requirements

QF - PCR

STR

3 -4  days

Amniotic Fluid,  3-4 ml Maternal Blood in EDTA,  CVS

 

Learn more:

Read: Uncover the Speed and Precision of QF-PCR Prenatal Testing

Listen: The Quick Benefits of QF-PCR for Prenatal Diagnosis

Watch: See How QF-PCR Revolutionizes Prenatal Testing

 

FAQs




What is QF-PCR testing?

QF-PCR (Quantitative Fluorescent Polymerase Chain Reaction) is a genetic test used to quickly and accurately detect common chromosomal abnormalities, such as those associated with Down syndrome, Edwards syndrome, and Patau syndrome.

When is QF-PCR testing recommended?

How is the sample collected for QF-PCR testing?

What conditions can QF-PCR detect?

How quickly can QF-PCR results be obtained?

Is QF-PCR as accurate as traditional karyotyping?

Can QF-PCR be used for postnatal testing?

Are there any risks associated with QF-PCR testing?

Is QF-PCR testing covered by insurance?

What should I do if the QF-PCR test results are abnormal?